Spheroids cultured for 2 days in Matrigel had several lumens inside associated with PKC zeta (yellow arrowheads) and ZO-1 accumulation (yellow arrows), ...

These cells showed clear apical-basal polarity in 2-D cultures. In 3-D cultures, however, each cell line displayed different responses to the same ECM. In MDCK ...

Spheroids of MDCK II, EpH4 and R2/7 α-Cate cells after 1.5–2 day culture in hanging drops. In this case, the ECM deposited by those cells is considered to be ...

Apical membranes are formed inside the spheroid. (C) Polarized epithelial cells forming a spheroid in suspension culture. Concentration of the ECM deposited by ...

Nov 13, 2014 · MDCK II, EpH4 and R2/7 α-Cate cells showed very clear apical-basal polarity in 2-D culture. But in 3-D culture, each cell type showed different ...

(a) A schematic representation of fluorescent apical and basolateral markers used in this study. (b) MDCK cells grown on filters were transfected with VSV-G–CFP ...

I am reaching out to this esteemed community seeking insights into a recent experiment I conducted involving cell seeding in agarose micro-wells.

... investigate the influence of substrate stiffness on apical-basal polarization in a 2D in vitro model, we cultured MDCKs on collagen-I coated substrates with ...