Dark Samus is referred to with female pronouns mainly in part due to possessing Samus's DNA, whereas Metroid Prime is referred to with gender-neutral pronouns. Oddly enough, an unused Logbook entry from Metroid Prime 3: Corruption refers to Dark Samus with gender-neutral pronouns as well.

Samus Aran (サムス・アラン, Samusu Aran) is the main character of the Metroid games. She's a bounty hunter, and helps the Galactic Federation. She has many enemies, including Mother Brain, Ridley, Kraid, Dark Samus, and the Metroids.

Samus broke ground early in the gaming world when she debuted in the 1986 game Metroid. Originally players were under the impression that Samus was a male, as the English translation of the instruction manual used male pronouns for her. However, completing Metroid in under five hours revealed Samus to be a young woman.

In Metroid: Other M, the Zero Suit is capable of materializing the Power Suit from within itself. She is 190 cm (6 ft 3 in) and 90 kilograms (200 lb) while wearing the Power Suit. The Super Metroid Nintendo's Player's Guide describes Samus as 6 ft 3 in (1.91 m) tall and weighs 198 pounds (90 kg) without her Power Suit.